Journal of Mass Spectrometry (Incorp Biological Mass Spectrometry) Wiley The purpose of this research was to investigate mechanisms of antiplatelet action of bioactive principle from S. Theoretical aspects of ion formation in the presence of ionic liquids both in LDI and MALDI analysis are discussed. MALDI‐MS thus can be used for the analysis of chemical reactions and the screening of enzyme‐catalyzed reactions in ionic liquids and for the analysis of the biocatalysts dissolved in these solvents. Relative quantitation of amino acids was possible using isotope‐labeled internal standards. Homogeneity of samples in the presence of ionic liquids was reduced compared with classical MALDI preparations. Optimal analysis conditions such as molar matrix‐to‐analyte and ionic liquid‐to‐matrix ratios were determined. Low molecular mass compounds and peptides could be analyzed best in the presence of water‐immiscible ionic liquids, whereas proteins gave the best results in water‐miscible ionic liquids. Sodium and potassium adducts were not observed in any analysis involving ionic liquids. Amino acids, peptides and proteins could be analyzed in ionic liquids after addition of matrix substances. In the latter case, adduct formation between anions and cations of the analytes was observed. Signals of both anions and cations of the ionic liquids could be observed both in LDI‐ and in MALDI‐MS. We characterized five different ionic liquids by laser desorption/ionization (LDI) and by matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry (MS) and studied the analysis of amino acids, peptides and proteins dissolved in these solvents. Ionic liquids are interesting solvents for a number of applications in chemistry and biotechnology. Zabet‐Moghaddam, Masoud Krüger, Ralf Heinzle, Elmar Tholey, Andreas Matrix‐assisted laser desorption/ionization mass spectrometry for the characterization of ionic liquids and the analysis of amino acids, peptides and proteins in ionic liquids Matrix‐assisted laser desorption/ionization mass spectrometry for the characterization of ionic.
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